Washington University in St Louis

The Preston M. Green Department of
Electrical & Systems Engineering

Journal covers

Correcting field-dependent aberrations with nanoscale accuracy in three-dimensional single-molecule localization microscopy
Optica Vol. 2, Iss. 11 (2015) Image credit: Matthew D. Lew
Rotational Mobility of Single Molecules Affects Localization Accuracy in Super-Resolution Fluorescence Microscopy
Nano Lett. Vol. 13, Iss. 9 (2013) Image credit: Matthew D. Lew
The double-helix microscope super-resolves extended biological structures by localizing single blinking molecules in three dimensions with nanoscale precision
Appl. Phys. Lett. Vol. 100, Iss. 15 (2012) Image credit: Matthew D. Lew

Previous Publications

Refereed Journal Publications

  1. O. Liba, M. D. Lew, E. D. SoRelle, R. Dutta, D. Sen, D. M. Moshfeghi, S. Chu, and A. de la Zerda, “Speckle-modulating optical coherence tomography in living mice and humans,” Nat. Commun. 8, 15845 (2017). [Highlight in Nat. Methods, EurekAlert!, Optics & Photonics News, Article]
  2. A. von Diezmann, M. Y. Lee, M. D. Lew, and W. E. Moerner, “Correcting field-dependent aberrations with nanoscale accuracy in three-dimensional single-molecule localization microscopy,” Optica 2, 985 (2015). [OSA news, Optics & Photonics News, Journal cover, Article]
  3. M. D. Lew, S. F. Lee, M. A. Thompson, H-L. D. Lee, and W. E. Moerner, “Single-molecule photocontrol and nanoscopy,” in Far-Field Optical Nanoscopy (eds. P. Tinnefeld, C. Eggeling, and S. W. Hell) 14, 87 (Springer-Verlag, 2015). [Book chapter]
  4. M. D. Lew and W. E. Moerner, “Azimuthal polarization filtering for accurate, precise, and robust single-molecule localization microscopy,” Nano Lett. 14, 6407 (2014). [Article]
  5. M. P. Backlund, M. D. Lew, A. S. Backer, S. J. Sahl, and W. E. Moerner, “The role of molecular dipole orientation in single-molecule fluorescence microscopy and implications for super-resolution imaging,” ChemPhysChem 15, 587 (2014). [Article]
  6. A. S. Backer, M. P. Backlund, M. D. Lew, and W. E. Moerner, “Single-molecule orientation measurements with a quadrated pupil,” Opt. Lett. 38, 1521 (2013). [Article]
  7. M. D. Lew*, M. P. Backlund*, and W. E. Moerner, “Rotational mobility of single molecules affects localization accuracy in super-resolution fluorescence microscopy,” Nano Lett. 13, 3967 (2013). [Journal cover, Article]
  8. M. P. Backlund*, M. D. Lew*, A. S. Backer, S. J. Sahl, G. Grover, A. Agrawal, R. Piestun, and W. E. Moerner, “Simultaneous, accurate measurement of the 3D position and orientation of single molecules,” Proc. Natl. Acad. Sci. USA 109, 19087 (2012). [Highlight in Nat. Methods, Article]
  9. M. A. Thompson, M. D. Lew, and W. E. Moerner, “Extending microscopic resolution with single-molecule imaging and active control,” Annu. Rev. Biophys. 41, 321 (2012). [Article]
  10. H-L. D. Lee*, S. J. Sahl*, M. D. Lew, and W. E. Moerner, “The double-helix microscope super-resolves extended biological structures by localizing single blinking molecules in three dimensions with nanoscale precision,” Appl. Phys. Lett. 100, 153701 (2012). [Journal cover, Article]
  11. M. D. Lew*, S. F. Lee*, J. L. Ptacin, M. K. Lee, R. J. Twieg, L. Shapiro, and W. E. Moerner, “Three-dimensional superresolution colocalization of intracellular protein superstructures and the cell surface in live Caulobacter crescentus,” Proc. Natl. Acad. Sci. USA 108, E1102 (2011). [Article]
  12. M. D. Lew, S. F. Lee, M. Badieirostami, and W. E. Moerner, “Corkscrew point spread function for far-field three-dimensional nanoscale localization of pointlike objects,” Opt. Lett. 36, 202 (2011). [Article]
  13. M. Badieirostami, M. D. Lew, M. A. Thompson, and W. E. Moerner, “Three-dimensional localization precision of the double-helix point spread function versus astigmatism and biplane,” Appl. Phys. Lett. 97, 161103 (2010). [Article]
  14. M. A. Thompson*, M. D. Lew*, M. Badieirostami, and W. E. Moerner, “Localizing and tracking single nanoscale emitters in three dimensions with high spatiotemporal resolution using a double-helix point spread function,” Nano Lett. 10, 211 (2010). [Article]
  15. X. Cui, M. Lew, and C. Yang, “Quantitative differential interference contrast microscopy based on structured-aperture interference,” Appl. Phys. Lett. 93, 091113 (2008). [Article]
  16. M. Lew, X. Cui, X. Heng, and C. Yang, “Interference of a four-hole aperture for on-chip quantitative two-dimensional differential phase imaging,” Opt. Lett. 32, 2963 (2007). [Article]

Other Publications

  1. A. S. Backer, M. P. Backlund, M. D. Lew, A. R. Diezmann, S. J. Sahl, and W. E. Moerner, “Single-molecule orientation measurements with a quadrated pupil,” Proc. SPIE 8950, 89500L (2014). [Article]
  2. M. D. Lew, A. R. S. von Diezmann, and W. E. Moerner, “Easy-DHPSF open-source software for three-dimensional localization of single molecules with precision beyond the optical diffraction limit,” Protocol Exchange (2013). [Article]
  3. M. P. Backlund*, M. D. Lew*, A. S. Backer, S. J. Sahl, G. Grover, A. Agrawal, R. Piestun, and W. E. Moerner, “The double-helix point spread function enables precise and accurate measurement of 3D single-molecule localization and orientation,” Proc. SPIE 8590, 85900L (2013). [Article]
  4. M. D. Lew, M. A. Thompson, M. Badieirostami, and W. E. Moerner, “In vivo three-dimensional superresolution fluorescence tracking using a double-helix point spread function,” Proc. SPIE 7571, 75710Z (2010). [Article]
  5. M. Lew, X. Cui, X. Heng, and C. Yang, “Two-dimensional differential interference contrast microscopy based on four-hole variation of Young's interference,” Proc. SPIE 6859, 685916 (2008). [Article]
  6. X. Cui, M. Lew, X. Heng, and C. Yang, “On-chip differential interference contrast (DIC) phase imager and beam profiler based on Young's interference,” Proc. SPIE 6441, 64411F (2007). [Article]
  7. M. Lew, X. Cui, and C. Yang, “Measuring the phase of light,” Caltech Undergraduate Research Journal 6, 18 (2007). [PDF - 831 KB]