Washington University in St Louis

The Preston M. Green Department of
Electrical & Systems Engineering

Journal covers

2024

  1. T. Wu and M. D. Lew, “Dipole-Spread Function Engineering for Six-Dimensional Super-Resolution Microscopy,” in Coded Optical Imaging (ed. J. Liang), 207 (Springer, 2024). [arXiv.org, Chapter]
  2. M. R. King, K. M. Ruff, A. Z. Lin, A. Pant, M. Farag, J. M. Lalmansingh, T. Wu, M. J. Fossat, W. Ouyang, M. D. Lew, E. Lundberg, M. D. Vahey, and R. V. Pappu, “Macromolecular condensation organizes nucleolar sub-phases to set up a pH gradient,” Cell 187, 1889 (2024). [The Source - Washington University, EurekAlert!, Article]
  3. W. Zhou, C. L. O’Neill, T. Ding, O. Zhang, J. S. Rudra, and M. D. Lew, “Resolving the Nanoscale Structure of β-Sheet Peptide Self-Assemblies Using Single-Molecule Orientation–Localization Microscopy,” ACS Nano 18, 8798 (2024). [Journal cover, Article, Data]
  4. T. Wu, W. Zhou, J. S. Rudra, R. V. Pappu, and M. D. Lew, “6D single-fluorogen orientation-localization microscopy for elucidating the architecture of beta-sheet assemblies and biomolecular condensates,” Proc. SPIE 12853, 1285302 (2024). [Open Scholarship, Article]
  5. J. Lu and M. D. Lew, “Single-molecule electrochemical imaging resolves the midpoint potentials of individual fluorophores on nanoporous antimony-doped tin oxide,” Chem. Sci. 15, 2037 (2024). [Journal cover, Article, Data]

2023

  1. O. Zhang, Z. Guo, Y. He, T. Wu, M. D. Vahey, and M. D. Lew, “Six-dimensional single-molecule imaging with isotropic resolution using a multi-view reflector microscope,” Nat. Photon. 17, 179 (2023). [The Source - Washington University, Open Scholarship, Article, Data, Video abstract]

2022

‡editor's pick/hot paper

  1. J. M. Jusuf and M. D. Lew, “Towards optimal point spread function design for resolving closely spaced emitters in three dimensions,” Opt. Express 30, 37154 (2022). [Article, Data]
  2. T. Wu, P. Lu, M. A. Rahman, X. Li, and M. D. Lew, “Deep-SMOLM: deep learning resolves the 3D orientations and 2D positions of overlapping single molecules with optimal nanoscale resolution,” Opt. Express 30, 36761 (2022). [McKelvey Engineering News, Article, Code, Data, Video abstract]
  3. M. Shen, T. Ding, C. Tan, W. H. Rackers, D. Zhang, M. D. Lew, and B. Sadtler, “In Situ Imaging of Catalytic Reactions on Tungsten Oxide Nanowires Connects Surface–Ligand Redox Chemistry with Photocatalytic Activity,” Nano Lett. 22, 4694 (2022). [Article]
  4. T. Wu, J. Lu, and M. D. Lew, “Dipole-spread-function engineering for simultaneously measuring the 3D orientations and 3D positions of fluorescent molecules,” Optica 9, 505 (2022). [The Source - Washington University, Article, Data, Video abstract]
  5. O. Zhang, W. Zhou, J. Lu, T. Wu, and M. D. Lew, “Resolving the three-dimensional rotational and translational dynamics of single molecules using radially and azimuthally polarized fluorescence,” Nano Lett. 22, 1024 (2022). [The Source - Washington University, Open Scholarship, Article, Data, Summary PDF, Video abstract]

2021

‡editor's pick/hot paper

  1. T. Ding and M. D. Lew, “Single-molecule localization microscopy of 3D orientation and anisotropic wobble using a polarized vortex point spread function,” J. Phys. Chem. B 125, 12718 (2021). [Journal cover, The Source - Washington University, EurekAlert!, Open Scholarship, Article, Data, Summary PDF]
  2. T. Ding and M. D. Lew, “Elucidating the nanoscale architecture of amyloid aggregates using a polarized donut point spread function,” Microsc. Microanal. 27 (S1), 1428 (2021). [Article]
  3. T. Wu, J. Lu, and M. D. Lew, “pixOL: pixel-wise point spread function engineering for measuring the 3D orientation and 3D location of dipole-like emitters,” Microsc. Microanal. 27 (S1), 858 (2021). [Article]
  4. M. Shen, T. Ding, W. H. Rackers, C. Tan, K. Mahmood, M. D. Lew, and B. Sadtler, “Single-molecule colocalization of redox reactions on semiconductor photocatalysts connects surface heterogeneity and charge-carrier separation in bismuth oxybromide,” J. Am. Chem. Soc. 143, 11393 (2021). [NSF-PAR, Article]
  5. M. D. Lew, “Computational Modelling Enables Robust Multidimensional Nanoscopy,” in Computational Modeling: From Chemistry to Materials to Biology (eds. K. Wüthrich, B. Weckhuysen, L. Rongy, and A. De Wit), 189 (World Scientific, 2021). [Open Scholarship, Article]
  6. O. Zhang and M. D. Lew, “Single-molecule orientation localization microscopy II: a performance comparison,” J. Opt. Soc. Am. A 38, 288 (2021). [arXiv.org, Article]
  7. O. Zhang and M. D. Lew, “Single-molecule orientation localization microscopy I: fundamental limits,” J. Opt. Soc. Am. A 38, 277 (2021). [arXiv.org, Article]

2020

‡editor's pick/hot paper

  1. H. Mazidi, T. Ding, A. Nehorai, and M. D. Lew, “Quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy,” Nat. Commun. 11, 6353 (2020). [The Source - Washington University, EurekAlert!, Article, Data, Summary PDF]
  2. ‡ J. Lu, H. Mazidi, T. Ding, O. Zhang, and M. D. Lew, “Single-Molecule 3D Orientation Imaging Reveals Nanoscale Compositional Heterogeneity in Lipid Membranes,” Angew. Chem. Int. Ed. 59, 17572 (2020). [Journal cover, The Source - Washington University, The Analytical Scientist, EurekAlert!, Open Scholarship, Article, Data]
  3. O. Zhang and M. D. Lew, “Quantum limits for precisely estimating the orientation and wobble of dipole emitters,” Phys. Rev. Research 2, 033114 (2020). [Article, Summary PDF]
  4. M. Shen, T. Ding, J. Luo, C. Tan, K. Mahmood, Z. Wang, D. Zhang, R. Mishra, M. D. Lew, and B. Sadtler, “Competing Activation and Deactivation Mechanisms in Photodoped Bismuth Oxybromide Nanoplates Probed by Single-Molecule Fluorescence Imaging,” J. Phys. Chem. Lett. 11, 5219 (2020). [NSF-PAR, Article]
  5. T. Ding*, T. Wu*, H. Mazidi, O. Zhang, and M. D. Lew, “Single-molecule orientation localization microscopy for resolving structural heterogeneities between amyloid fibrils,” Optica 7, 602 (2020). [OSA news release, The Source - Washington University, EurekAlert!, Photonics Media, Article, Data, Summary PDF]
  6. T. Wu, T. Ding, H. Mazidi, O. Zhang, and M. D. Lew, “A computationally-efficient bound for the variance of measuring the orientation of single molecules,” Proc. SPIE 11246, 1124616 (2020). [Open Scholarship, Article]
  7. H. Mazidi, T. Ding, A. Nehorai, and M. D. Lew, “Measuring localization confidence for quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy,” Proc. SPIE 11246, 1124611 (2020). [Open Scholarship, Article]
  8. M. Shen*, T. Ding*, S. T. Hartman, F. Wang, C. Krucylak, Z. Wang, C. Tan, B. Yin, R. Mishra, M. D. Lew, and B. Sadtler, “Nanoscale Colocalization of Fluorogenic Probes Reveals Role of Oxygen Vacancies in the Photocatalytic Activity of Tungsten Oxide Nanowires,” ACS Catal. 10, 2088 (2020). [Open Scholarship, Article]

2019

  1. H. Mazidi, E. S. King, O. Zhang, A. Nehorai, and M. D. Lew, “Dense Super-Resolution Imaging of Molecular Orientation via Joint Sparse Basis Deconvolution and Spatial Pooling,” 2019 IEEE 16th International Symposium on Biomedical Imaging (ISBI 2019), 325 (2019). [arXiv.org, Article]
  2. O. Zhang and M. D. Lew, “Fundamental Limits on Measuring the Rotational Constraint of Single Molecules using Fluorescence Microscopy,” Phys. Rev. Lett. 122, 198301 (2019). [The Source - Washington University, arXiv.org, Article, Summary PDF]
  3. O. Zhang and M. D. Lew, “Fundamental limits of measuring single-molecule rotational mobility,” Proc. SPIE 10884, 1088412 (2019). [Open Scholarship, Article]
  4. T. Ding, K. Spehar, J. Bieschke, and M. D. Lew, “Long-term, super-resolution imaging of amyloid structures using transient amyloid binding microscopy,” Proc. SPIE 10884, 108840J (2019). [Open Scholarship, Article]

2018

  1. H. Mazidi, J. Lu, A. Nehorai, and M. D. Lew, “Minimizing Structural Bias in Single-Molecule Super-Resolution Microscopy,” Sci. Rep. 8, 13133 (2018). [Article, Data, Summary PDF]
  2. D. Maji, J. Lu, P. Sarder, A. H. Schmieder, G. Cui, X. Yang, D. Pan, M. D. Lew, S. Achilefu, and G. M. Lanza “Cellular Trafficking of Sn-2 Phosphatidylcholine Prodrugs Studied with Fluorescence Lifetime Imaging and Super-resolution Microscopy,” Prec. Nanomed. 1, 128 (2018). [Journal cover, Article]
  3. K. Spehar*, T. Ding*, Y. Sun, N. Kedia, J. Lu, G. R. Nahass, M. D. Lew, and J. Bieschke, “Super-Resolution Imaging of Amyloid Structures over Extended Times Using Transient Binding of Single Thioflavin T Molecules,” ChemBioChem 19, 1944 (2018). [Journal cover, The Source - Washington University, NSF Science360, Futurity, EurekAlert!, OSA News Release, Open Scholarship, Article, Summary PDF]
  4. O. Zhang, J. Lu, T. Ding, and M. D. Lew, “Imaging the three-dimensional orientation and rotational mobility of fluorescent emitters using the Tri-spot point spread function,” Appl. Phys. Lett. 113, 031103 (2018). [Open Scholarship, Article, Summary PDF]
    Correction: Appl. Phys. Lett. 115, 069901 (2019). [Article]
  5. H. Mazidi, A. Nehorai, and M. D. Lew, “A robust statistical estimation (RoSE) algorithm jointly recovers the 3D location and intensity of single molecules accurately and precisely,” Proc. SPIE 10500, 105000E (2018). [Open Scholarship, Article]
  6. O. Zhang, T. Ding, J. Lu, H. Mazidi, and M. D. Lew, “Measuring 3D molecular orientation and rotational mobility using a Tri-spot point spread function,” Proc. SPIE 10500, 105000B (2018). [Open Scholarship, Article]