Journal covers








2025
-
M. D. Lew. “Painting rich six-dimensional pictures using
polarized fluorescence microscopy,”
Proc. Natl. Acad. Sci. U.S.A. 122, e2501914122
(2025). [Open Scholarship,
Article]
-
T. Wu*, M. R. King*, Y. Qiu, M. Farag, R. V. Pappu, and M. D. Lew.
“Single-fluorogen imaging reveals distinct environmental and
structural features of biomolecular condensates,”
Nat. Phys. (2025). [The Source - Washington University,
EurekAlert!,
Article, Data]
-
Y. Chen, Y. Qiu, and M. D. Lew, “Resolving the Orientations
of and Angular Separation Between a Pair of Dipole
Emitters,” Phys. Rev. Lett. 134, 093805
(2025). [McKelvey Engineering News,
arXiv,
Article]
2024
-
O. Zhang, and M. D. Lew, “Single-molecule
orientation-localization microscopy: Applications and
approaches,” Quarterly Reviews of Biophysics
57, e17 (2024). [Article]
-
E. Bruggeman, O. Zhang, L.-M. Needham, M. Körbel, S. Daly, M.
Cheetham, R. Peters, T. Wu, A. S. Klymchenko, S. J. Davis, E. K.
Paluch, D. Klenerman, M. D. Lew, K. O’Holleran, and S. F. Lee,
“POLCAM: Instant Molecular Orientation Microscopy for the
Life Sciences,” Nat. Methods 21, 1873 (2024).
[Article]
-
W. Zhou, T. Wu, and M. D. Lew, “Fundamental Limits in
Measuring the Anisotropic Rotational Diffusion of Single
Molecules,” J. Phys. Chem. A 128, 5808 (2024).
[Journal cover,
The Source - Washington University,
Open Scholarship,
Article, Data]
-
B. Sun, T. Ding, W. Zhou, T. S. Porter, and M. D. Lew,
“Single-Molecule Orientation Imaging Reveals the
Nano-Architecture of Amyloid Fibrils Undergoing Growth and
Decay,” Nano Lett. 24, 7276 (2024). [The Source - Washington University,
EurekAlert!,
Open Scholarship,
Article, Data]
-
T. Wu and M. D. Lew, “Dipole-Spread Function Engineering for
Six-Dimensional Super-Resolution Microscopy,” in
Coded Optical Imaging (ed. J. Liang), 207 (Springer, 2024).
[arXiv.org,
Chapter]
-
M. R. King, K. M. Ruff, A. Z. Lin, A. Pant, M. Farag, J. M.
Lalmansingh, T. Wu, M. J. Fossat, W. Ouyang, M. D. Lew, E.
Lundberg, M. D. Vahey, and R. V. Pappu, “Macromolecular
condensation organizes nucleolar sub-phases to set up a pH
gradient,” Cell 187, 1889 (2024). [The Source - Washington University,
EurekAlert!,
Article]
-
W. Zhou, C. L. O’Neill, T. Ding, O. Zhang, J. S. Rudra, and M. D.
Lew, “Resolving the Nanoscale Structure of β-Sheet Peptide
Self-Assemblies Using Single-Molecule Orientation–Localization
Microscopy,” ACS Nano 18, 8798 (2024). [Journal cover,
The Source - Washington University,
EurekAlert!,
Open Scholarship,
Article, Data]
-
T. Wu, W. Zhou, J. S. Rudra, R. V. Pappu, and M. D. Lew, “6D
single-fluorogen orientation-localization microscopy for
elucidating the architecture of beta-sheet assemblies and
biomolecular condensates,” Proc. SPIE 12853,
1285302 (2024). [Open Scholarship,
Article]
-
J. Lu and M. D. Lew, “Single-molecule electrochemical
imaging resolves the midpoint potentials of individual
fluorophores on nanoporous antimony-doped tin oxide,”
Chem. Sci. 15, 2037 (2024). [Journal cover,
Article, Data]
2023
-
O. Zhang, Z. Guo, Y. He, T. Wu, M. D. Vahey, and M. D. Lew,
“Six-dimensional single-molecule imaging with isotropic
resolution using a multi-view reflector microscope,”
Nat. Photon. 17, 179 (2023). [The Source - Washington University,
Open Scholarship,
Article, Data,
Video abstract]
2022
‡editor's pick/hot paper
-
‡
J. M. Jusuf and M. D. Lew, “Towards optimal point spread
function design for resolving closely spaced emitters in three
dimensions,” Opt. Express 30, 37154 (2022).
[Article, Data]
-
T. Wu, P. Lu, M. A. Rahman, X. Li, and M. D. Lew,
“Deep-SMOLM: deep learning resolves the 3D orientations and
2D positions of overlapping single molecules with optimal
nanoscale resolution,” Opt. Express 30, 36761
(2022). [McKelvey Engineering News,
Article,
Code, Data,
Video abstract]
-
M. Shen, T. Ding, C. Tan, W. H. Rackers, D. Zhang, M. D. Lew, and
B. Sadtler, “In Situ Imaging of Catalytic Reactions
on Tungsten Oxide Nanowires Connects Surface–Ligand Redox
Chemistry with Photocatalytic Activity,” Nano Lett.
22, 4694 (2022). [NSF-PAR,
Article]
-
T. Wu, J. Lu, and M. D. Lew, “Dipole-spread-function
engineering for simultaneously measuring the 3D orientations and
3D positions of fluorescent molecules,” Optica
9, 505 (2022). [The Source - Washington University,
Article, Data,
Video abstract]
-
O. Zhang, W. Zhou, J. Lu, T. Wu, and M. D. Lew, “Resolving
the three-dimensional rotational and translational dynamics of
single molecules using radially and azimuthally polarized
fluorescence,” Nano Lett. 22, 1024 (2022). [The Source - Washington University,
EurekAlert!,
Open Scholarship,
Article, Data,
Summary PDF,
Video abstract]
2021
‡editor's pick/hot paper
-
T. Ding and M. D. Lew, “Single-molecule localization
microscopy of 3D orientation and anisotropic wobble using a
polarized vortex point spread function,”
J. Phys. Chem. B 125, 12718 (2021). [Journal cover,
The Source - Washington University,
EurekAlert!,
Open Scholarship,
Article, Data,
Summary PDF]
-
T. Ding and M. D. Lew, “Elucidating the nanoscale
architecture of amyloid aggregates using a polarized donut point
spread function,” Microsc. Microanal. 27 (S1),
1428 (2021). [Article]
-
T. Wu, J. Lu, and M. D. Lew, “pixOL: pixel-wise point spread
function engineering for measuring the 3D orientation and 3D
location of dipole-like emitters,”
Microsc. Microanal. 27 (S1), 858 (2021). [Article]
-
M. Shen, T. Ding, W. H. Rackers, C. Tan, K. Mahmood, M. D. Lew,
and B. Sadtler, “Single-molecule colocalization of redox
reactions on semiconductor photocatalysts connects surface
heterogeneity and charge-carrier separation in bismuth
oxybromide,” J. Am. Chem. Soc. 143, 11393
(2021). [NSF-PAR,
Article]
-
M. D. Lew, “Computational Modelling Enables Robust
Multidimensional Nanoscopy,” in
Computational Modeling: From Chemistry to Materials to
Biology
(eds. K. Wüthrich, B. Weckhuysen, L. Rongy, and A. De Wit), 189
(World Scientific, 2021). [Open Scholarship,
Article]
-
‡
O. Zhang and M. D. Lew, “Single-molecule orientation
localization microscopy II: a performance comparison,”
J. Opt. Soc. Am. A 38, 288 (2021). [arXiv.org,
Article]
-
O. Zhang and M. D. Lew, “Single-molecule orientation
localization microscopy I: fundamental limits,”
J. Opt. Soc. Am. A 38, 277 (2021). [arXiv.org,
Article]
2020
‡editor's pick/hot paper
-
H. Mazidi, T. Ding, A. Nehorai, and M. D. Lew, “Quantifying
accuracy and heterogeneity in single-molecule super-resolution
microscopy,” Nat. Commun. 11, 6353 (2020). [The Source - Washington University,
EurekAlert!,
Article, Data,
Summary PDF]
-
‡ J. Lu, H. Mazidi, T. Ding, O. Zhang, and M. D. Lew,
“Single-Molecule 3D Orientation Imaging Reveals Nanoscale
Compositional Heterogeneity in Lipid Membranes,”
Angew. Chem. Int. Ed. 59, 17572 (2020). [Journal cover,
The Source - Washington University,
The Analytical Scientist,
EurekAlert!,
Open Scholarship,
Article, Data]
-
O. Zhang and M. D. Lew, “Quantum limits for precisely
estimating the orientation and wobble of dipole emitters,”
Phys. Rev. Research 2, 033114 (2020). [Article, Summary PDF]
-
M. Shen, T. Ding, J. Luo, C. Tan, K. Mahmood, Z. Wang, D. Zhang,
R. Mishra, M. D. Lew, and B. Sadtler, “Competing Activation
and Deactivation Mechanisms in Photodoped Bismuth Oxybromide
Nanoplates Probed by Single-Molecule Fluorescence Imaging,”
J. Phys. Chem. Lett. 11, 5219 (2020). [NSF-PAR,
Article]
-
T. Ding*, T. Wu*, H. Mazidi, O. Zhang, and M. D. Lew,
“Single-molecule orientation localization microscopy for
resolving structural heterogeneities between amyloid
fibrils,” Optica 7, 602 (2020). [OSA news release,
The Source - Washington University,
EurekAlert!,
Photonics Media,
Article, Data,
Summary PDF]
-
T. Wu, T. Ding, H. Mazidi, O. Zhang, and M. D. Lew, “A
computationally-efficient bound for the variance of measuring the
orientation of single molecules,” Proc. SPIE
11246, 1124616 (2020). [Open Scholarship,
Article]
-
H. Mazidi, T. Ding, A. Nehorai, and M. D. Lew, “Measuring
localization confidence for quantifying accuracy and heterogeneity
in single-molecule super-resolution microscopy,”
Proc. SPIE 11246, 1124611 (2020). [Open Scholarship,
Article]
-
M. Shen*, T. Ding*, S. T. Hartman, F. Wang, C. Krucylak, Z. Wang,
C. Tan, B. Yin, R. Mishra, M. D. Lew, and B. Sadtler,
“Nanoscale Colocalization of Fluorogenic Probes Reveals Role
of Oxygen Vacancies in the Photocatalytic Activity of Tungsten
Oxide Nanowires,” ACS Catal. 10, 2088 (2020).
[Open Scholarship,
Article]
2019
-
H. Mazidi, E. S. King, O. Zhang, A. Nehorai, and M. D. Lew,
“Dense Super-Resolution Imaging of Molecular Orientation via
Joint Sparse Basis Deconvolution and Spatial Pooling,”
2019 IEEE 16th International Symposium on Biomedical Imaging
(ISBI 2019), 325 (2019). [arXiv.org,
Article]
-
O. Zhang and M. D. Lew, “Fundamental Limits on Measuring the
Rotational Constraint of Single Molecules using Fluorescence
Microscopy,” Phys. Rev. Lett. 122, 198301
(2019). [The Source - Washington University,
arXiv.org,
Article, Summary PDF]
-
O. Zhang and M. D. Lew, “Fundamental limits of measuring
single-molecule rotational mobility,” Proc. SPIE
10884, 1088412 (2019). [Open Scholarship,
Article]
-
T. Ding, K. Spehar, J. Bieschke, and M. D. Lew, “Long-term,
super-resolution imaging of amyloid structures using transient
amyloid binding microscopy,” Proc. SPIE 10884,
108840J (2019). [Open Scholarship,
Article]
2018
-
H. Mazidi, J. Lu, A. Nehorai, and M. D. Lew, “Minimizing
Structural Bias in Single-Molecule Super-Resolution
Microscopy,” Sci. Rep. 8, 13133 (2018). [Article, Data,
Summary PDF]
- D. Maji, J. Lu, P. Sarder, A. H. Schmieder, G. Cui, X. Yang, D. Pan, M. D. Lew, S. Achilefu, and G. M. Lanza “Cellular Trafficking of Sn-2 Phosphatidylcholine Prodrugs Studied with Fluorescence Lifetime Imaging and Super-resolution Microscopy,” Prec. Nanomed. 1, 128 (2018). [Journal cover, Article]
-
K. Spehar*, T. Ding*, Y. Sun, N. Kedia, J. Lu, G. R. Nahass, M. D.
Lew, and J. Bieschke, “Super-Resolution Imaging of Amyloid
Structures over Extended Times Using Transient Binding of Single
Thioflavin T Molecules,” ChemBioChem 19, 1944
(2018). [Journal cover,
The Source - Washington University,
NSF Science360,
Futurity,
EurekAlert!,
OSA News Release,
Open Scholarship,
Article, Summary PDF]
-
O. Zhang, J. Lu, T. Ding, and M. D. Lew, “Imaging the
three-dimensional orientation and rotational mobility of
fluorescent emitters using the Tri-spot point spread
function,” Appl. Phys. Lett. 113, 031103
(2018). [Open Scholarship,
Article, Summary PDF]
Correction: Appl. Phys. Lett. 115, 069901 (2019). [Article]
-
H. Mazidi, A. Nehorai, and M. D. Lew, “A robust statistical
estimation (RoSE) algorithm jointly recovers the 3D location and
intensity of single molecules accurately and precisely,”
Proc. SPIE 10500, 105000E (2018). [Open Scholarship,
Article]
-
O. Zhang, T. Ding, J. Lu, H. Mazidi, and M. D. Lew,
“Measuring 3D molecular orientation and rotational mobility
using a Tri-spot point spread function,” Proc. SPIE
10500, 105000B (2018). [Open Scholarship,
Article]