Journal covers
![Anisotropic rotational diffusion of single molecules, represented here by magenta dipoles wobbling within cyan elliptical cones, can be measured using a fluorescence microscope. However, photon shot noise within these images (bottom) causes each measured cone to vary in shape. (Image credit: Matthew D. Lew)](../images/JPCA_cover_2024_Zhou_sm.webp)
![Single-molecule orientation localization microscopy captures fluorescence from Nile red molecules as they transiently bind to self-assembled fibrils composed of engineered KFE8 peptides. We observe considerable polymorphism across the network of interconnected fibrils, revealed by the orientations and wobble of individual Nile red, with nanoscale resolution. (Image credit: Weiyan Zhou)](../images/ACSnano_cover_2024_Zhou_sm.webp)
![Showcasing research from Professor Matthew D. Lew's laboratory, McKelvey School of Engineering, Washington University in St. Louis, MO, USA. Single-molecule electrochemical imaging resolves the midpoint potentials of individual fluorophores on nanoporous antimony-doped tin oxide.](../images/ChemSci_cover_2024_Lu_sm.webp)
![W. E. Moerner Festschrift Virtual Special Issue](../images/JPCB_cover_2022_Lew_sm.webp)
![Single-Molecule Localization Microscopy of 3D Orientation and Anisotropic Wobble Using a Polarized Vortex Point Spread Function](../images/JPCB_cover_2021_Ding_sm.webp)
![Single‐Molecule 3D Orientation Imaging Reveals Nanoscale Compositional Heterogeneity in Lipid Membranes](../images/AngewChemie_cover_2020_Lu_sm.webp)
![Super-resolution Imaging of Amyloid Structures over Extended Times by Using Transient Binding of Single Thioflavin T Molecules](../images/ChemBioChem_cover_2018_Spehar_Ding_sm.webp)
![Cellular Trafficking of Sn-2 Phosphatidylcholine Prodrugs Studied with Fluorescence Lifetime Imaging and Super-resolution Microscopy](../images/PrecNanomed_cover_2018_Maji_sm.webp)
2024
- W. Zhou, T. Wu, and M. D. Lew, “Fundamental Limits in Measuring the Anisotropic Rotational
Diffusion of Single Molecules,” J. Phys. Chem. A 128, 5808 (2024). [Journal cover, The Source -
Washington University, Open
Scholarship, Article, Data]
- B. Sun, T. Ding, W. Zhou, T. S. Porter, and M. D. Lew, “Single-Molecule Orientation
Imaging Reveals the Nano-Architecture of Amyloid Fibrils Undergoing Growth and Decay,”
Nano Lett. 24, 7276
(2024). [The
Source - Washington University, EurekAlert!, Open
Scholarship, Article, Data]
- T. Wu and M. D. Lew, “Dipole-Spread Function Engineering for Six-Dimensional
Super-Resolution Microscopy,” in Coded Optical Imaging (ed. J. Liang), 207
(Springer, 2024). [arXiv.org,
Chapter]
- M. R. King, K. M. Ruff, A. Z. Lin, A. Pant, M. Farag, J. M. Lalmansingh, T. Wu, M. J. Fossat, W.
Ouyang, M. D. Lew, E. Lundberg, M. D. Vahey, and R. V. Pappu, “Macromolecular condensation
organizes nucleolar sub-phases to set up a pH gradient,” Cell 187, 1889
(2024). [The
Source - Washington University, EurekAlert!, Article]
- W. Zhou, C. L. O’Neill, T. Ding, O. Zhang, J. S. Rudra, and M. D. Lew, “Resolving the
Nanoscale Structure of β-Sheet Peptide Self-Assemblies Using Single-Molecule
Orientation–Localization Microscopy,” ACS Nano 18, 8798 (2024). [Journal cover, The
Source - Washington University, EurekAlert!, Open
Scholarship, Article, Data]
- T. Wu, W. Zhou, J. S. Rudra, R. V. Pappu, and M. D. Lew, “6D single-fluorogen
orientation-localization microscopy for elucidating the architecture of beta-sheet assemblies
and biomolecular condensates,” Proc. SPIE 12853, 1285302 (2024). [Open
Scholarship, Article]
- J. Lu and M. D. Lew, “Single-molecule electrochemical imaging resolves the midpoint
potentials of individual fluorophores on nanoporous antimony-doped tin oxide,” Chem.
Sci. 15, 2037 (2024). [Journal cover, Article, Data]
2023
- O. Zhang, Z. Guo, Y. He, T. Wu, M. D. Vahey, and M. D. Lew, “Six-dimensional
single-molecule imaging with isotropic resolution using a multi-view reflector
microscope,” Nat. Photon. 17, 179 (2023). [The
Source - Washington University, Open Scholarship, Article, Data, Video abstract]
2022
‡editor's pick/hot paper
- ‡ J. M. Jusuf and M. D.
Lew, “Towards optimal point spread function design for resolving closely spaced emitters
in three dimensions,” Opt. Express 30, 37154 (2022). [Article, Data]
- T. Wu, P. Lu, M. A. Rahman, X. Li, and M. D. Lew, “Deep-SMOLM: deep learning resolves the
3D orientations and 2D positions of overlapping single molecules with optimal nanoscale
resolution,” Opt. Express 30, 36761 (2022). [McKelvey
Engineering News, Article, Code, Data, Video abstract]
- M. Shen, T. Ding, C. Tan, W. H. Rackers, D. Zhang, M. D. Lew, and B. Sadtler, “In
Situ Imaging of Catalytic Reactions on Tungsten Oxide Nanowires Connects Surface–Ligand
Redox Chemistry with Photocatalytic Activity,” Nano Lett. 22, 4694 (2022).
[NSF-PAR, Article]
- T. Wu, J. Lu, and M. D. Lew, “Dipole-spread-function engineering for simultaneously
measuring the 3D orientations and 3D positions of fluorescent molecules,” Optica
9, 505 (2022). [The
Source - Washington University, Article, Data, Video abstract]
- O. Zhang, W. Zhou, J. Lu, T. Wu, and M. D. Lew, “Resolving the three-dimensional
rotational and translational dynamics of single molecules using radially and azimuthally
polarized fluorescence,” Nano Lett. 22, 1024 (2022). [The
Source - Washington University, EurekAlert!, Open
Scholarship, Article, Data, Summary
PDF, Video
abstract]
2021
‡editor's pick/hot paper
- T. Ding and M. D. Lew, “Single-molecule localization microscopy of 3D orientation and
anisotropic wobble using a polarized vortex point spread function,” J. Phys. Chem.
B 125, 12718 (2021). [Journal cover, The
Source - Washington University, EurekAlert!, Open
Scholarship, Article, Data, Summary
PDF]
- T. Ding and M. D. Lew, “Elucidating the nanoscale architecture of amyloid aggregates using
a polarized donut point spread function,” Microsc. Microanal. 27 (S1), 1428
(2021). [Article]
- T. Wu, J. Lu, and M. D. Lew, “pixOL: pixel-wise point spread function engineering for
measuring the 3D orientation and 3D location of dipole-like emitters,” Microsc.
Microanal. 27 (S1), 858 (2021). [Article]
- M. Shen, T. Ding, W. H. Rackers, C. Tan, K. Mahmood, M. D. Lew, and B. Sadtler,
“Single-molecule colocalization of redox reactions on semiconductor photocatalysts
connects surface heterogeneity and charge-carrier separation in bismuth oxybromide,” J.
Am. Chem. Soc. 143, 11393 (2021). [NSF-PAR, Article]
- M. D. Lew, “Computational Modelling Enables Robust Multidimensional Nanoscopy,” in
Computational Modeling: From Chemistry to Materials to Biology (eds. K. Wüthrich, B.
Weckhuysen, L. Rongy, and A. De Wit), 189 (World Scientific, 2021). [Open Scholarship, Article]
- ‡ O. Zhang and M.
D. Lew, “Single-molecule orientation localization microscopy II: a performance
comparison,” J. Opt. Soc. Am. A 38, 288 (2021). [arXiv.org, Article]
- O. Zhang and M. D. Lew, “Single-molecule orientation localization microscopy I:
fundamental limits,” J. Opt. Soc. Am. A 38, 277 (2021). [arXiv.org, Article]
2020
‡editor's pick/hot paper
- H. Mazidi, T. Ding, A. Nehorai, and M. D. Lew, “Quantifying accuracy and heterogeneity in
single-molecule super-resolution microscopy,” Nat. Commun. 11, 6353 (2020).
[The
Source - Washington University, EurekAlert!, Article, Data, Summary
PDF]
- ‡ J. Lu, H. Mazidi, T. Ding, O. Zhang, and M. D. Lew, “Single-Molecule 3D Orientation
Imaging Reveals Nanoscale Compositional Heterogeneity in Lipid Membranes,” Angew. Chem.
Int. Ed. 59, 17572 (2020). [Journal cover, The
Source - Washington University, The Analytical
Scientist, EurekAlert!,
Open
Scholarship, Article, Data]
- O. Zhang and M. D. Lew, “Quantum limits for precisely estimating the orientation and
wobble of dipole emitters,” Phys. Rev. Research 2, 033114 (2020). [Article, Summary PDF]
- M. Shen, T. Ding, J. Luo, C. Tan, K. Mahmood, Z. Wang, D. Zhang, R. Mishra, M. D. Lew, and B.
Sadtler, “Competing Activation and Deactivation Mechanisms in Photodoped Bismuth
Oxybromide Nanoplates Probed by Single-Molecule Fluorescence Imaging,” J. Phys. Chem.
Lett. 11, 5219 (2020). [NSF-PAR, Article]
- T. Ding*, T. Wu*, H. Mazidi, O. Zhang, and M. D. Lew, “Single-molecule orientation
localization microscopy for resolving structural heterogeneities between amyloid fibrils,”
Optica 7, 602 (2020). [OSA
news release, The
Source - Washington University, EurekAlert!, Photonics
Media, Article,
Data, Summary
PDF]
- T. Wu, T. Ding, H. Mazidi, O. Zhang, and M. D. Lew, “A computationally-efficient bound for
the variance of measuring the orientation of single molecules,” Proc. SPIE
11246, 1124616 (2020). [Open Scholarship, Article]
- H. Mazidi, T. Ding, A. Nehorai, and M. D. Lew, “Measuring localization confidence for
quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy,”
Proc. SPIE 11246, 1124611 (2020). [Open Scholarship, Article]
- M. Shen*, T. Ding*, S. T. Hartman, F. Wang, C. Krucylak, Z. Wang, C. Tan, B. Yin, R. Mishra, M.
D. Lew, and B. Sadtler, “Nanoscale Colocalization of Fluorogenic Probes Reveals Role of
Oxygen Vacancies in the Photocatalytic Activity of Tungsten Oxide Nanowires,” ACS
Catal. 10, 2088 (2020). [Open Scholarship, Article]
2019
- H. Mazidi, E. S. King, O. Zhang, A. Nehorai, and M. D. Lew, “Dense Super-Resolution
Imaging of Molecular Orientation via Joint Sparse Basis Deconvolution and Spatial
Pooling,” 2019 IEEE 16th International Symposium on Biomedical Imaging (ISBI 2019),
325 (2019). [arXiv.org, Article]
- O. Zhang and M. D. Lew, “Fundamental Limits on Measuring the Rotational Constraint of
Single Molecules using Fluorescence Microscopy,” Phys. Rev. Lett. 122,
198301 (2019). [The
Source - Washington University, arXiv.org, Article, Summary PDF]
- O. Zhang and M. D. Lew, “Fundamental limits of measuring single-molecule rotational
mobility,” Proc. SPIE 10884, 1088412 (2019). [Open Scholarship, Article]
- T. Ding, K. Spehar, J. Bieschke, and M. D. Lew, “Long-term, super-resolution imaging of
amyloid structures using transient amyloid binding microscopy,” Proc. SPIE
10884, 108840J (2019). [Open Scholarship, Article]
2018
- H. Mazidi, J. Lu, A. Nehorai, and M. D. Lew, “Minimizing Structural Bias in
Single-Molecule Super-Resolution Microscopy,” Sci. Rep. 8, 13133 (2018). [Article, Data, Summary
PDF]
- D. Maji, J. Lu, P. Sarder, A. H. Schmieder, G. Cui, X. Yang, D. Pan, M. D. Lew, S. Achilefu, and G. M. Lanza “Cellular Trafficking of Sn-2 Phosphatidylcholine Prodrugs Studied with Fluorescence Lifetime Imaging and Super-resolution Microscopy,” Prec. Nanomed. 1, 128 (2018). [Journal cover, Article]
- K. Spehar*, T. Ding*, Y. Sun, N. Kedia, J. Lu, G. R. Nahass, M. D. Lew, and J. Bieschke,
“Super-Resolution Imaging of Amyloid Structures over Extended Times Using Transient
Binding of Single Thioflavin T Molecules,” ChemBioChem 19, 1944 (2018). [Journal cover, The Source -
Washington University, NSF
Science360, Futurity, EurekAlert!, OSA
News Release, Open Scholarship, Article, Summary PDF]
- O. Zhang, J. Lu, T. Ding, and M. D. Lew, “Imaging the three-dimensional orientation and
rotational mobility of fluorescent emitters using the Tri-spot point spread function,”
Appl. Phys. Lett. 113, 031103 (2018). [Open Scholarship, Article, Summary PDF]
Correction: Appl. Phys. Lett. 115, 069901 (2019). [Article]
- H. Mazidi, A. Nehorai, and M. D. Lew, “A robust statistical estimation (RoSE) algorithm
jointly recovers the 3D location and intensity of single molecules accurately and
precisely,” Proc. SPIE 10500, 105000E (2018). [Open Scholarship, Article]
- O. Zhang, T. Ding, J. Lu, H. Mazidi, and M. D. Lew, “Measuring 3D molecular orientation
and rotational mobility using a Tri-spot point spread function,” Proc. SPIE
10500, 105000B (2018). [Open Scholarship, Article]