Washington University in St Louis

The Preston M. Green Department of
Electrical & Systems Engineering

Welcome to the Lew Lab archives! Here you'll find noteworthy milestones and past lab news. See how far the lab has come.

2024

Dipole-Spread Function Engineering for Six-Dimensional Super-Resolution MicroscopyTingting's review chapter on dipole-spread function engineering for six-dimensional imaging, part of a new book, Coded Optical Imaging, is now online!
Read it here: “Dipole-Spread Function Engineering for Six-Dimensional Super-Resolution Microscopy

Congratulations to our close colleague Dr. Matt King in the Pappu lab on his study of pH gradients within the nucleolus, now appearing in Cell!
- Read it here: “Macromolecular condensation organizes nucleolar sub-phases to set up a pH gradient
- News coverage: The Source - Washington University, EurekAlert!

APS logoMatt gave an invited talk in the “Quantum Sensing and Information Processing for Biomedical Applications” session at the APS March Meeting in Minneapolis, entitled “6D Single-Molecule Orientation-Localization Microscopy: Fundamental Limits for Visualizing the Dynamic Organization of Biomolecules.”

Single-molecule electrochemical imaging resolves spatially varying midpoint potentials on a transparent electrodeCongratulations to Prof. Jin Lu, now at the National Center for Nanoscience, whose work on single-molecule electrochemical imaging is now online in Chemical Science!
- Read it here: “Single-Molecule Electrochemical Imaging Resolves the Midpoint Potentials of Individual Fluorophores on Nanoporous Antimony-Doped Tin Oxide
Showcasing research from Professor Matthew D. Lew's laboratory, McKelvey School of Engineering, Washington University in St. Louis, MO, USA. Single-molecule electrochemical imaging resolves the midpoint potentials of individual fluorophores on nanoporous antimony-doped tin oxide.- Update: Jin's article is now featured as an inside cover in Volume 15, Issue 6 of Chem. Sci.!

SPIE Photonics West logoMatt gave an invited talk, “6D single-fluorogen orientation-localization microscopy for elucidating the architecture of beta-sheet assemblies and biomolecular condensates,” in the “High-Speed Biomedical Imaging and Spectroscopy IX” conference at Photonics West in San Francisco. [Open Scholarship, Article]

Single-Molecule Orientation-Localization Microscopy article screenshotOumeng's news article, “Single-molecule orientation-localization microscopy,” is now online in Imaging & Microscopy.

2023

Lew Lab group photo 2023

Tingting's PhD defense, November 20, 2023Tingting successfully defended her thesis, “Information-Rich 6D Single-Molecule Imaging Using Optimization,” on Monday, November 20, at 1 PM in Green Hall's Rodin Auditorium. Congratulations and we will miss you, Tingting!

Lew Lab alternate photo 2023 We hiked together and celebrated Tingting's many contributions to the group. After graduating, she will be a Senior Optical Metrology Engineer at Metalenz in Boston, MA. Thank you for bringing advanced computation and biomolecular condensates to the lab!

2nd annual SPECTRA student-led conference flyerBrian presenting his talkBrian presenting his talk Brian and Jasmine presented their research at the 2nd annual SPECTRA student-led conference. Both the talks and poster session had superb attendance. Thanks to the wonderful 2023 officers for bringing together the WashU optics community!

SERMACS 2023, Durham, NC, October 25-28, 2023 Matt presented “Single-fluorogen imaging reveals the nanoscale structure of beta-sheet assemblies and biomolecular condensates” in the “Single-molecule Dynamics in Complex Chemical and Biological Systems” session at SERMACS 2023.

Capturing images of molecules with a new level of resolution: An interview with Prof. Matthew Lew” was published in Wiley Analytical Science magazine.

Gordon Research Conferences Chemical Imaging 2023 Poster Award to Weiyan Zhou Congratulations to Weiyan, who won a poster award at the Chemical Imaging GRC 2023!

Our lab has received a five-year $2 million Maximizing Investigators’ Research Award (MIRA) from the National Institutes of Health (NIH) to develop adaptive six-dimensional microscopy. Congrats, all!

Tingting Wu with Imaging Science Program Co-Director Joe Culver Congratulations, Tingting, on receiving a 2023 Outstanding Research Award from the Imaging Science Doctoral Program!

Congratulations, Jasmine, on receiving a 2023 Summer Undergraduate Research Award!

Jasmine Cheng's photoBrian Sun's photo Two outstanding undergraduates have received awards this year: Brian with the Rick Grodsky Award for Technical Achievement and the David H. Levy Outstanding Senior Award, and Jasmine with the Russel R. Pfeiffer Outstanding Junior Award! Both were recognized at the 2023 McKelvey Engineers' Honors Ceremony. Congratulations, Brian and Jasmine!

2023 OFSA Winners with Provost Beverly Wendland and Vice Provost Vijay Ramani. Photo credit: Ron Klein. Matt has received a 2023 Outstanding Faculty Award from the Graduate Student Senate. The awardees were recognized at a ceremony organized by the GSS. Thank you, students, for this honor!

BPS 2023 logo The Lew Lab presented several exciting posters and talks at BPS 2023 in San Diego:
- Congratulations to Tingting, who has received a 2023 Travel Award to present at the Annual Meeting!
Weiyan's submission to the 2023 Biophysical Society Art of Science Image Contest - Congratulations to Weiyan, whose submission to the Biophysical Society Art of Science Image Contest was selected as one of ten finalists! Her art was displayed in the Exhibit Hall during the meeting.
- Weiyan presented “Single-Molecule Orientation-Localization Microscopy Resolves the Nanoscale Organization of Self-Assembled Peptides,” Poster 627-Pos / B448 in Optical Microscopy and Superresolution Imaging I on Sunday, Feb 19 [Abstract]
- Brian presented “Single-Molecule Orientation-Localization Microscopy Resolves How Amyloidophilic Dye Orientations are Correlated with Amyloid Fiber Growth and Disruption,” Poster 1339-Pos / B468 in Optical Microscopy and Superresolution Imaging II on Monday, Feb 20 [Abstract]
- Matt presented “A Multi-View Reflector Microscope Visualizes Six-Dimensional Single-Molecule Translational And Rotational Dynamics With Isotropic Nanoscale Resolution,” Poster 1351-Pos / B480 in Optical Microscopy and Superresolution Imaging II on Monday, Feb 20 [Abstract]
- Tingting presented “Mapping Inhomogeneous Network Structures within Biomolecular Condensate using Single-Molecule Imaging and Tracking of Fluorogenic Probes,” Platform Talk 1437-Plat in Condensates: Physical Properties and Modeling II on Tuesday, Feb 21 at 9:15 AM [Abstract]

2022

Lew Lab group photo 2022

Congratulations to Oumeng and our collaborators in the Vahey Lab in WashU Biomedical Engineering on publishing a new microscope architecture for 6D single-molecule super-resolution imaging in Nature Photonics! The multi-view reflector (MVR) microscope achieves nearly isotropic 10.9 nm precision in 3D over a 1.5 μm depth range and measures the 3D orientation of single molecules with 2.0° precision.
- “Six-dimensional single-molecule imaging with isotropic resolution using a multi-view reflector microscope,” Nat. Photon. 17, 179 (2023). [The Source - Washington University, Open Scholarship, Article, Data, Summary PDF]
- “Telescope-inspired microscope sees molecules in 6D” in the Source.

Deep-SMOLM: deep learning resolves the 3D orientations and 2D positions of overlapping single molecules with optimal nanoscale resolution Tingting's collaborative work with Peng and Asheq on using deep-learning for 5D imaging (2D position and 3D orientation estimation) of single molecules is now online in Optics Express. Congratulations!
- “Deep-SMOLM: deep learning resolves the 3D orientations and 2D positions of overlapping single molecules with optimal nanoscale resolution,” Opt. Express 30, 36761 (2022). [McKelvey Engineering News, Article, Code, Data, Video abstract]
- “Machine learning generates pictures of proteins in 5D” in McKelvey Engineering News.

Matt introducing the Single Molecules and Phase session Matt led the “Single Molecules and Phase” session at the 65th Welch Conference on Chemical Research, “Molecules and Sculpted Light.” W. E. Moerner chaired the conference, which featured awesome science and timely addresses from Peter Hotez and Carolyn R. Bertozzi.

Center for Biomolecular Condensates LogoRohit introducing Matt at the symposium The Lew Lab is thrilled to be part of the new Center for Biomolecular Condensates in the McKelvey School of Engineering. The inaugural symposium was inspirational and great fun! [McKelvey Engineering News]

Towards optimal point spread function design for resolving closely spaced emitters in three dimensions James's theoretical exploration of how to design microscopes optimized for resolving closely spaced objects in 3D is now online in Optics Express. Congratulations!
- “Towards optimal point spread function design for resolving closely spaced emitters in three dimensions,” Opt. Express 30, 37154 (2022). [Article]
- James's paper has been highlighted as an Editor's Pick!

In Situ Imaging of Catalytic Reactions on Tungsten Oxide Nanowires Connects Surface–Ligand Redox Chemistry with Photocatalytic Activity Dr. Meikun Shen, formerly in the Sadtler group, has published a study on how ascorbic acid enhances water oxidation activity on the surfaces of tungsten oxide nanowires. It appears in Nano Lett. Congratulations!
In Situ Imaging of Catalytic Reactions on Tungsten Oxide Nanowires Connects Surface–Ligand Redox Chemistry with Photocatalytic Activity” [Article]

Can a Computer Help Make a Better Camera? Matt is spotlighted as a STEM expert in the summer 2022 issue of the Saint Louis Science Center's NewScience magazine. He discusses the incredible potential of computational imaging in “Can a Computer Help Make a Better Camera?”.
Read: Magazine version, Web/mobile version, PDF [51.8 MB]

Congratulations, Oumeng, on being featured as an outstanding graduate by the McKelvey School of Engineering!

Jasmine Cheng's photo Congratulations, Jasmine, on receiving a 2022 Summer Undergraduate Research Award!

Dipole-spread-function engineering for simultaneously measuring the 3D orientations and 3D positions of fluorescent molecules Tingting's work on engineering new dipole-spread functions (DSFs) for imaging single molecules in 6 dimensions (3D position plus 3D orientation) is now online in Optica. Congratulations!
- “Dipole-spread-function engineering for simultaneously measuring the 3D orientations and 3D positions of fluorescent molecules,” Optica 9, 505 (2022). [Article, Data, Video abstract]
- “Lew lab sheds new light on cell membranes” - The Source

Yuanxin's portraitYiyang's portrait The lab welcomes Yiyang Chen and Yuanxin Qiu, both in the Imaging Science PhD program. It's great to have you!

Matt give another invited colloquium, “Single-Molecule Orientation Localization Microscopy: Visualizing Molecular Organization at the Nanoscale”, at the Department of Physics & Astronomy at San Francisco State University.

Matt give an invited talk, “Single-Molecule Orientation Localization Microscopy: Visualizing Molecular Organization at the Nanoscale”, at the MIT Laser Biomedical Research Center's Modern Optics and Spectroscopy Seminar.

Congratulations to Dr. Hesam Mazidi, whose patent on using Wasserstein-induced flux to certify localization accuracy has been granted!

Tingting presented “pixOL: pixel-wise dipole-spread function engineering for simultaneously measuring the 3D orientation and 3D location of dipole-like emitters” at Focus on Microscopy 2022.

Oumeng successfully defends his PhD thesis on April 7, 2022 Congratulations, Dr. Oumeng Zhang, on successfully defending his PhD dissertation!

We congratulate Oumeng on receiving the Department of Electrical and Systems Engineering's Outstanding Doctoral Dissertation Award! Thank you for your outstanding contributions to the technology and culture of innovation in our lab!

We also congratulate Brian on receiving the Russell R. Pfeiffer Outstanding Junior Award! It is well-deserved!

The Washington University in St. Louis Board of Trustees has promoted Matt to the rank of Associate Professor with tenure. Onward and upward!

Tingting receives a second-place poster award at the 2022 WashU Imaging Sciences Pathway Retreat Congratulations Tingting on receiving a second-place poster award at the 2022 Imaging Sciences Pathway Retreat!

Tingting gives a flash talk at BPS 2022 in San Francisco, CA The Lew Lab presented several posters at BPS 2022 in San Francisco:
- “Accurate Superresolution Microscopy of the 3D Orientations and 3D Locations of Single Molecules within Lipid Membranes and Biomolecular Condensates,” Poster 694-Pos / B518 in Optical Microscopy and Superresolution Imaging I [Abstract]
- “Visualizing the Nanoscale Architecture of Amyloid Aggregates using Amyloidophilic Probes and a Polarized Vortex Microscope,” Poster 693-Pos / B517 in Optical Microscopy and Superresolution Imaging I [Abstract]
- “Mapping the Chemical Composition and Nanoscale Structure of Lipid Membranes using Polarized Fluorescence from Single Molecules,” Poster 2353-Pos / B183 in Membrane Structure II [Abstract]

Tribute to W. E. Moerner Today, the Lew Lab celebrates the science and mentorship of W. E. Moerner and the publication of a Festschrift issue of the Journal of Physical Chemistry dedicated to him. Thank you, W. E., for your pioneering work and leadership that continues to inspire generations of scientists and engineers!
Read:
- Tribute to W. E. Moerner
- Autobiography of W. E. Moerner
- About the cover
- Our contribution to the Festschrift: “Single-Molecule Localization Microscopy of 3D Orientation and Anisotropic Wobble Using a Polarized Vortex Point Spread Function

Welcome, Wentao, to the Lew Lab!

A radially and azimuthally polarized microscope resolves rotational dynamics of Nile red within lipid bilayers Oumeng's study of using radially and azimuthally polarized fluorescence to measure the translational and rotational dynamics of Nile red in lipid bilayers is now online in Nano Letters. Congratulations!
- “Resolving the Three-Dimensional Rotational and Translational Dynamics of Single Molecules Using Radially and Azimuthally Polarized Fluorescence,” Nano Lett. 22, 1024 (2022). [Open Scholarship, Article, Summary PDF]
- “Lew lab sheds new light on cell membranes” - The Source

2021

Lew Lab group photo 2021

The polarized vortex PSF resolves single-molecule wobble in 3D Tianben's postdoctoral work on developing the polarized vortex PSF for imaging the 3D orientations and 3D wobble of single molecules is now online in J. Phys. Chem. B. Congratulations!
“Single-Molecule Localization Microscopy of 3D Orientation and Anisotropic Wobble Using a Polarized Vortex Point Spread Function,” J. Phys. Chem. B 125, 12718 (2021). [The Source - Washington University, EurekAlert!, Open Scholarship, Article, Summary PDF

Update: Tianben's art has been selected as a supplemental cover for volume 125, issue 46 of J. Phys. Chem. B!

Matt at NA-ISMET 2021 Matt gave a talk about single-molecule imaging of electron transfer pathways at NA-ISMET 2021 in Los Angeles, CA. There were many fascinating talks covering all aspects of microbial electrochemistry and technology. Thanks to Moh and his group for organizing and hosting!

Single-Molecule Approaches to Chemistry and Biology Matt gave an invited talk in the “Single-Molecule Approaches to Chemistry and Biology” session at SERMACS 2021 in Birmingham, AL. It was great to see such fantastic single-molecule studies in person! Thanks Kevin for organizing!

Farewell celebration for Tianben We got together for a beautiful hike and celebrated Tianben's many contributions to the group. We wish him well as a new researcher at Hitachi, Ltd. in Japan. Thank you for inspiring us with new optical technologies and studies on catalysis and amyloid imaging!

Tianben and Tingting gave platform presentations at Microscopy & Microanalysis 2021 Tianben and Tingting gave platform presentations at the Microscopy and Microanalysis 2021 Virtual Meeting, Symposium B11.3 - Frontiers in Fluorescence Lifetime and Super-resolution Imaging of Biological Structures and Dynamics.

Congratulations, Tingting, on being designated a 2021 M&M Student Scholar!

“Elucidating the nanoscale architecture of amyloid aggregates using a polarized donut point spread function” [MP4 - 54 MB]
“pixOL: pixel-wise point spread function engineering for measuring the 3D orientation and 3D location of dipole-like emitters” [MP4 - 38 MB]

Colocalization of Redox Reactions on Semiconductor PhotocatalystsDr. Meikun Shen, formerly in the Sadtler group, has published another study on surface heterogeniety and charge-carrier separation in semiconductor photocatalysts. It appears in J. Am. Chem. Soc. Congratulations!
“Single-Molecule Colocalization of Redox Reactions on Semiconductor Photocatalysts Connects Surface Heterogeneity and Charge-Carrier Separation in Bismuth Oxybromide” [Article]

Matt was designated as an OSA Senior Member. Congratulations!

Matt presented at the 2021 OSA Imaging and Applied Optics Congress:

“Robustly Detecting Imaging Model Mismatches and Reconstruction Artifacts in Single-Molecule Localization Microscopy” [MP4 - 57 MB]
“Imaging Chemical Environments and Amyloid Architectures using Single-Molecule Orientation-Localization Microscopy” [MP4 - 120 MB]

Probing chemical reactions by single-molecule spectroscopy speakers photo Matt spoke at the “Probing Chemical Reactions by Single-Molecule Spectroscopy” virtual conference. It was a fascinating day of single-molecule research! Thank you Suzanne and Randy for organizing!

Makaiya photoZiyi photoWeiyan photo The lab extends a (belated) welcome to Weiyan, Ziyi, and Makaiya! Weiyan Zhou has joined our lab for her PhD studies. Ziyi Hu and Makaiya Smith are working with us on summer research projects. Learn more about them.

2021 graduation 2021 farewell to Jin After more than a year apart, the group celebrated Hesam's and Tianben's accomplishments. Congratulations on earning your PhDs!
We also wished Jin farewell as he begins his new position at the National Center for Nanoscience and Technology in Beijing. Thank you for making us all better chemists!

Tingting Wu-Outstanding Graduate Student Assistant to the Instructor Award Congratulations to Tingting on receiving the department's Outstanding Graduate Student Assistant to the Instructor (AI) Award! Tingting received this award during the McKelvey Engineering Honor Ceremony held on April 21, 2021.

Focus on Microscopy 2021 online conference Oumeng and Matt presented talks at Focus on Microscopy 2021 online, March 28-31:
- “Measuring Single-Molecule Orientations Using a Radially and Azimuthally Polarized Epifluorescence Microscope”
- “Visualizing Membrane Composition and Amyloid Aggregate Organization Using Single-Molecule 3D Orientation Imaging”

The lab welcomes Brian Sun, who has received a Summer Undergraduate Research Award to work with us this summer on amyloid imaging. Congratulations Brian!

A radially and azimuthally polarized imaging system achieves superior orientation estimation performance Oumeng's two-part series exploring orientation-localization microscopy has been accepted to J. Opt. Soc. A!
- “Single-molecule orientation localization microscopy I: fundamental limits” [arXiv.org, Article]
- “Single-molecule orientation localization microscopy II: a performance comparison” [arXiv.org, Article]
And, the SMOLM II paper has been selected as an “Editors' Pick”! Congratulations, Oumeng!

Matt gave an invited seminar “Toward Visualizing the Dynamic Organization of Amyloid Aggregates using Single-Molecule Orientation Localization Microscopy” at the Knight Alzheimer Disease Research Center at Washington University.

“Toward Visualizing the Dynamic Organization of Amyloid Aggregates using Single-Molecule Orientation Localization Microscopy” [MP4 - 328 MB]

2020

Lew Lab group photo 2020

WIF effectively denoises inaccurate fits from any localization software Hesam's development and characterization of Wasserstein Induced Flux for certifying the statistical confidence of localization microscopy datasets is now online in Nature Communications! Congratulations, Hesam!
Read the press coverage: The Source - Washington University, EurekAlert!
Read the article: “Quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy”

Matt has been honored with the 2020 “Excellence in Teaching” award from Emerson Electric Co.! Each year, honorees are chosen by the deans of their schools and recognized by the Center for Teaching and Learning.

Tianben, Jin, and Oumeng presented their recent work at S3IC 2020: Single-Molecule Sensors and NanoSystems. Check out their presentations here:

“Orientations of transiently binding Nile red molecules reveal the organization of amyloid aggregates,” presented at S3IC 2020 [MP4 - 54.2 MB]
“Single-molecule orientation imaging for probing lipid nanodomains,” presented at S3IC 2020 [MP4 - 37.9 MB]
“Precision limits for measuring the 3D orientations of fluorescent molecules,” presented at S3IC 2020 [MP4 - 27.4 MB]

Congratulations to Oumeng, whose patent covering the tri-spot PSF has been granted!

Polar angle and wobble maps of Nile red molecules in lipid nanodomains Single-molecule orientation localization microscopy in lipid bilayers Jin's study using the orientational tumbling of Nile red to infer the chemical composition of lipid bilayers is now online in Angewandte Chemie.
Update: The paper has been selected by the editors as a “Hot Paper” on Nanoscale Sensors. Congratulations, Jin!
Read the press coverage: The Source - Washington University, The Analytical Scientist, EurekAlert!
Read the article: “Single-molecule 3D orientation imaging reveals nanoscale compositional heterogeneity in lipid membranes”

, updated 8.3.20

Congratulations to Dr. Hesam Mazidi, Dr. Tianben Ding, and Jack Hyde on successfully defending their dissertations! It's been a pleasure having you in the lab!

An interferometric imaging system for achieving the Quantum Cramer-Rao BoundOumeng's work on deriving the quantum limits of measuring molecular orientation is now online in Phys. Rev. Research. Congratulations, Oumeng!
“Quantum limits for precisely estimating the orientation and wobble of dipole emitters” [Article]

Colocalization of fluorogenic probes on tungsten oxide nanowiresMeikun in the Sadtler group has published another study on the photocatalytic activity of semiconductor nanoplates in J. Phys. Chem. Lett. Congratulations!
“Competing Activation and Deactivation Mechanisms in Photodoped Bismuth Oxybromide Nanoplates Probed by Single-Molecule Fluorescence Imaging” [Article]

Single-molecule orientation localization microscopy of amyloid fibrilsTianben and Tingting's work on using super-resolution single-molecule orientation localization microscopy to study amyloid aggregates is now online in Optica. Congratulations!
See: single-molecule orientation localization microscopy in action
Read the press coverage: OSA news release, The Source - Washington University, EurekAlert!, Photonics Media
Read the article: “Single-molecule orientation localization microscopy for resolving structural heterogeneities between amyloid fibrils”

James portraitTristan portraitWe welcome Tristan Carlson and James Cevasco to the lab!

Max portraitCongratulations to Max on his Summer Undergraduate Research Award! Max will be working with Jack on our ultrafast camera collaboration with the Buckley lab in Physics.

Matt has been recognized with the 2019-2020 Outstanding Teaching Award from the Department of Electrical and Systems Engineering! Matt received the award as part of the ESE Awards and Recognition Ceremony on May 8, 2020.

Jack portraitCongratulations to Jack for winning the Rick Grodsky ESE Award for Technical Achievement! Jack received the award as part of the ESE Awards and Recognition Ceremony on May 8, 2020.

Matt and Oumeng presented at the Computational Optical Imaging workshop at the IEEE ISBI 2020 virtual conference.

“Fundamental quantum bounds on the precision and accuracy of measuring molecular orientation” [MP4 - 33.1 MB]
“Quantifying and Maximizing Imaging Accuracy in Single-Molecule Super-Resolution Microscopy” [MP4 - 183 MB]

Colocalization of fluorogenic probes on tungsten oxide nanowiresOur collaborative work on imaging photocatalytic activity, jointly led by Tianben and Meikun in the Sadtler group in the Department of Chemistry, is now online in ACS Catalysis. Congratulations, all!
“Nanoscale Colocalization of Fluorogenic Probes Reveals Role of Oxygen Vacancies in the Photocatalytic Activity of Tungsten Oxide Nanowires” [Open Scholarship, Article]
Press coverage: The Source - Washington University

Jin and Tianben presented their recent work at the 64th Annual Meeting of the Biophysical Society in San Diego.

Hesam and Tingting presented their recent work in the “Single Molecule Spectroscopy and Superresolution Imaging XIII” session at SPIE Photonics West in San Francisco. Read about their work here:
“Measuring localization confidence for quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy” [Open Scholarship, Article]
“A computationally-efficient bound for the variance of measuring the orientation of single molecules” [Open Scholarship, Article]

Quantitative BioImaging ConferenceHesam's work on “Quantifying Localization Accuracy in Single-Molecule Super-Resolution Microscopy” was presented in the “New Frontiers in Single Molecule Microscopy” session at QBI 2020 in Oxford, UK.

2019

Lew Lab group photo 2019 Alternate photo

25th Solvay Conference on ChemistryMatt gave an invited talk in the “Computational Modeling in High-Resolution Imaging” session at the 25th Solvay Conference on Chemistry “Computational Modeling: From Chemistry to Materials to Biology” in Brussels, Belgium.

Oumeng's paper entitled “Fundamental limits on measuring the rotational constraint of single molecules using fluorescence microscopy” has been published in Physical Review Letters. Congratulations! [arXiv.org, Article]
Press coverage: The Source - Washington University

Oumeng's and Hesam's works were presented at the OSA Imaging and Applied Optics Congress on June 24-27, 2019, in Munich:
Fundamental Limits on Imaging the Orientational Dynamics of Dipole-Like Emitters
Single-Molecule Super-Resolution Imaging of Molecular Orientation using a Tri-Spot Point Spread Function

Tara portraitJames portraitThe lab welcomes James Jusuf and Tara Porter to St. Louis! Both James and Tara were awarded Summer Undergraduate Research Fellowships from Caltech to lead their own research projects in our group.

Imaging Sciences Pathway retreat 2019Matt gave an invited talk at Washington University's Imaging Sciences Pathway Retreat 2019 entitled “Single Molecules and Point Spread Function Engineering for Visualizing Amyloid Protein Dynamics at the Nanoscale.” The group also presented several posters at the event!

BSC 2019Matt gave an invited talk at the 5th Annual Meeting of the Biophysical Society of Canada at the University of Toronto, Mississauga.

2019 farewell partyThe group threw a farewell board game party for Eshan, Kevin, and Weiran. Thank you all for being wonderful colleagues, and we wish you the best in your next endeavors!

Eshan presented his and Hesam's work on DNA imaging at the IEEE International Symposium on Biomedical Imaging (ISBI) on Apr. 8-11, 2019, in Venice.
“Dense Super-Resolution Imaging of Molecular Orientation Via Joint Sparse Basis Deconvolution and Spatial Pooling” [arXiv.org, Article]

Oumeng and Kevin presented their work at the OSA Biophotonics Congress: Optics in the Life Sciences meeting on Apr. 14-17, 2019, in Tuscon.
Measuring Rotational Dynamics with High Accuracy and Precision Using a Tri-spot Point Spread Function
Long-Term Super-Resolution Imaging of Amyloid Structures Using Transient Binding of Thioflavin T” [OSA News Release]

Our lab has begun working with a multi-university team to build functional living electronics, called Livtronics, as part of the Office of Naval Research-funded Multidisciplinary University Research Initiative (MURI) Program. Our aim is to build new fluorescence imaging technologies to visualize electron transport within living systems. Read more about it here. [McKelvey School of Engineering news]

Tianben and Oumeng presented their work at Photonics West. See it here:
“Long-term, super-resolution imaging of amyloid structures using transient amyloid binding microscopy”
“Fundamental limits of measuring single-molecule rotational mobility”

Tingting WuTingting Wu, a PhD student in our Imaging Science PhD program, has joined the Lew Lab. Welcome, Tingting!

2018

Lew Lab group photo 2018

Jack SURA 2018 posterJack presented his summer research project at WashU's Fall 2018 Undergraduate Research Symposium.

Kevin's and Tianben's article on super-resolution transient amyloid binding (TAB) microscopy is a featured cover article at ChemBioChem. Congratulations to the entire team! Read it here:
“Super-Resolution Imaging of Amyloid Structures over Extended Times Using Transient Binding of Single Thioflavin T Molecules” [Open Scholarship]
Press coverage: The Source - Washington University, NSF Science360, Futurity, EurekAlert!

Henry CohenHenry Cohen, who is studying for an MS degree in Biomedical Engineering, has joined the Lew Lab and received a Master's Fellowship. Welcome, Henry!

Hesam's article on the characteristics of structural bias in super-resolution microscopy and how to minimize it using our RoSE algorithm is now online at Scientific Reports. Congratulations! Read it here:
“Minimizing Structural Bias in Single-Molecule Super-Resolution Microscopy”

Weiran WangWeiran Wang, who is studying for an MS degree in Electrical Engineering, has joined the Lew Lab and received a Master's Fellowship. Welcome, Weiran!

Precision Nanomedicine cover, Issue 1(2)Our collaborative work with Dr. Greg Lanza on imaging the intracellular fate of lipid prodrugs is a featured cover article in Precision Nanomedicine. Read it here:
“Cellular Trafficking of Sn-2 Phosphatidylcholine Prodrugs Studied with Fluorescence Lifetime Imaging and Super-resolution Microscopy”

Oumeng's article on the Tri-spot point spread function is now online at Applied Physics Letters. Congratulations Oumeng! Read it here:
“Imaging the three-dimensional orientation and rotational mobility of fluorescent emitters using the Tri-spot point spread function”
[Open Scholarship]

Matt Stanford OSA seminar 2018Matt gave an invited Stanford Optical Society seminar entitled “Computational Optics for Multidimensional Nanoscale Imaging of Single Fluorescent Molecules.”

Jack HydeJack Hyde, who is majoring in Electrical Engineering, has joined the Lew Lab. He has received a Summer Undergraduate Research Award from the Office of Undergraduate Research. Welcome, Jack!

Kevin SpeharKevin Spehar, who is majoring in Biomedical Engineering, has joined the Lew Lab. Welcome, Kevin!

Hesam and Oumeng gave excellent presentations at SPIE BiOS (Photonics West). See them here:
“A robust statistical estimation (RoSE) algorithm jointly recovers the 3D location and intensity of single molecules accurately and precisely”
“Measuring 3D molecular orientation and rotational mobility using a Tri-spot point spread function”

2017

Lew Lab group photo 2017

Matthew Lew and Jan BieschkeMatt has received a five-year, $1.6 million Maximizing Investigators' Research Award from the National Institute of General Medical Sciences, part of the National Institutes of Health. The project is entitled "Five-Dimensional Single-Molecule Nanoscopy for Sensing and Imaging the Dynamic Functions of Biomolecules." We are collaborating with Jan Bieschke at UCL's Institute of Prion Diseases. Congratulations team! [The Source - Washington University]

Eshan SURA 2017 posterEshan presented his summer research project at the WashU Fall 2017 Undergraduate Research Symposium.

Hesam's and Oumeng's abstracts have been accepted for oral presentations in the "Single Molecule Spectroscopy and Superresolution Imaging XI" conference at SPIE BiOS (Photonics West). We're looking forward to showcasing our work in San Francisco in 2018! [Conference program]

Eshan KingEshan King, who is majoring in Biomedical Engineering and Applied Mathematics, has joined the Lew Lab. He has received a Summer Undergraduate Research Award from the Office of Undergraduate Research. Welcome, Eshan!

We have received a Collaboration Initiation Grant from the School of Engineering & Applied Science to build a fluorescence-lifetime imaging nanoscope in collaboration with James Buckley in the Department of Physics. Congratulations team! [WashU Engineering News]

Oumeng has successfully defended his Master's thesis, "Measuring Molecular Orientation and Rotational Mobility Using a Tri-spot Point Spread Function." Congratulations Oumeng! [ESE MS Defense Announcement PDF]

Matt receives an NSF CAREER AwardMatt has received a five-year, $500,000 CAREER Award from the National Science Foundation for the project entitled "CAREER: Nanoscale sensing and imaging using computational single-molecule nanoscopy." Congratulations team! [WashU Engineering News, The Source - Washington University]

2016

Lew Lab group photo 2016

Jin LuDr. Jin Lu joined the Lew Lab. Welcome Jin!

Our joint project "Single-Molecule, Super-Resolution Imaging of Reaction Sites in Mesoscale Semiconductor Photocatalysts" with Prof. Bryce Sadtler in the Department of Chemistry has been funded by the WashU International Center for Advanced Renewable Energy and Sustainability (I-CARES)!

Hiruma/Wagner Award plaqueMatt was awarded the Hiruma/Wagner Award at the 16th Conference of Peace through Mind/Brain Science.

We imaged single fluorescent molecules for the first time! What beautiful blinking!

2015

Tianben DingTianben Ding, a PhD student in Electrical and Systems Engineering, joined the Lew Lab. Welcome, Tianben!

Optics & Photonics News Photo Contest 2015Matt won first place in Optics and Photonics News's 10th annual After Image photo contest!

Matt gave an invited talk at Laser Science 2015 entitled “Accurate 3D nanoscale imaging of dipole-like emitters.”

Lab construction is completeLab construction is completed and ready for equipment move-in!

Matt gave the first lecture of ESE 582/BME 5820, Fundamentals and Applications of Modern Optical Imaging, a new graduate course at Washington University.

Matt joined the the Department of Electrical and Systems Engineering at Washington University in St. Louis as an Assistant Professor.